Comparison of allele-specific expression in Sistani cattle and its crossbreed with Holstein, Simmental, and Montbeliarde breeds

The present study has analyzed the allelic-specific expression in Purebred Sistani (Bos Indicus) and their crossbreed with Holstein, Simmental, and Montbeliarde breeds (Bos Taurus). The blood samples were taken from the caudal vein of purebred Sistani cows and crossbreed Sistani's with Holstein, Simental, and Montbeliarde (4 treatments). We discovered 152,496 (Purebred Sistani), 134,285 (Sistani × Simmental), 163,362 (Sistani × Montbeliarde), and 177,042 (Sistani × Holstein) SNPs on the assembled transcriptomes. In the Purebred Sistani, 8295 (5%), Sistani × Holstein crossbreed 11,900 (7%), Sistani × Simmental crossbreed 13,187 (10%), and Sistani × Montbeliarde crossbreed 16,666 (10%) number of SNPs were identified as ASE-SNPs. In the present study, 12 SNPs types identify, of which four were transition and eight were transversion. The most common SNPs were transition types. These SNPs were present in purebred Sistani 71.84%, Sistani × Holstein crossbreed 72.65%, Sistani × Simmental crossbreed 72.60%, and Sistani × Montbeliarde crossbreed 71.94%. Ontology analysis of the expressed genes in these cows revealed the involvement of these genes in different Biological classifications. Conducting such studies in parts of the world, such as the Sistan region, where it is not possible to record accurate records of cows, is a suitable and economical method for identifying genes with different expressions.